GENEQ > Biotechnology > Chloride Analyzers

Chloride Analyzer
Model 926S


Determination of chloride in sweat for diagnosis of fibrosis Cystic.

Clinical measurement of the chloride ion is essential for monitoring of electrolyte balance of patients as it plays, in combination with sodium and potassium, an important part in maintaining water balance between cells, plasma and interstitial fluid. The Model 926s is also widely used in the confirmation of the diagnosis of Cystic Fibrosis. The model 926S variant can accept 20 microlitre samples.
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PRINCIPLES OF METHOD

The main difficulties with the classic method were that silver nitrate reagent would change on storage due to a photochemical reaction and that the titration end point is visually weak. The reagent would have to be standardized requiring a highly skilled analytical technician

The 926 method is based on a coulometric titration and is an absolute method where the reagent, the silver ions, is precisely and quantitatively generated at the time of the analysis by passing a constant current between donor electrodes. The end point is detected by the use of sensing electrodes which measure the change in solution conductivity which occurs when excess silver ions are present in solution.

REAGENT SYSTEM
The Buffer system is optimised to eliminate interfering ions and incorporates a special dispersant to ensure reproducible formation of the silver chloride precipitate and enhance analytical performance.

SPECIFICATIONS
 
Model 926
Model 926S
Sample size
500 microlitres
20 microlitres
Readout Range
10-999mg Cl/l; 2-165mg%Salt
10-299mmolCl/l
Analysis Time
35 sec @ 200mgl/l
35 sec@ 100mmolCl/l
Accuracy
SD <1% @ 0.1% Cl Std Solution
+-2% @ NQCS 100mmol/l
Linearity
1% in range 10-999mgCl/l
1% 10-299 mmolCl/l
Reproducibility CV for 20 replicates
<1.5% @ 200mgCl/l
<1.5% @ 100mmolCl/l

REAGENT SYSTEM

The  Buffer system is optimised to eliminate interfering ions and incorporates a special dispersant to ensure reproducible formation of the silver chloride precipitate and enhance analytical performance.


THE SHERWOOD ADVANTAGE
    • The coulometric method is very robust and versatile
    • The Sherwood microprocessor based Instrument is ergonomically designed and easy to use and maintain
    • The Sherwood Buffer System specifically designed for the model 926 gives outstanding sensitivity and selectivity