GENEQ > Biotechnology > Microbiology

Detection of Listeria Monocytogenes

AxSys Remote Station

FEATURES
  • FAST - 24 hours total test time
  • ENRICHMENT FREE - No time-consuming, biohazard-generating enrichment needed
  • SENSITIVE - Equal to cultural methods, but in only 24 hours
  • ECONOMICAL - Inexpensive and easy to use
  • QUANTITATIVE - Detect Listeria as CFU/g

Microbiological contamination is a serious problem facing the food industry today, and Listeria monocytogenes is one of the most hazardous microbes. From 1986-1989, FDA Class I recalls for Listeria affected 52,000 pounds of seafood, 55 million sandwiches, 12,000 pounds of meat, and over 860 million pounds of cheese.

ListerTest helps detect and control Listeria by making testing of foods and the processing environment easy and rapid. ListerTest detects injured Listeria. It also eliminates enrichment, which is not only time consuming, but can produce false negatives if the Listeria are killed by harsh selective agents. And, ListerTest won't produce false positives either - eliminating the need and cost of confirmation. In our test, Listeria are bound to antibodies on microscopic magnetic beads. These beads are then isolated from the rest of the food or environmental sample. Listeria are confirmed immunologically and, if desired, caracterized biochemically. All in about 24 hours.

Best of all, because the rest is simple, it can be run in house. No other Listeria test is this fast, simple or sensitive.

HERE'S HOW SIMPLE LISTERTEST IS
  • Add ListerTest Beads to a prepared sample to capture live Listeria
  • Spread beads on a plate
  • After 22 hours, examine plate for colonies:
    If no colonies appear, the result is negative and the test is done
    If colonies appear, lift them onto a membrane, then quantitate Listeria immunologically and, if desired, caracterize biochemically.

CAT. NO. DESCRIPTION
Kits and bulk test option
LIS-330-000 Lift kit environment (20 tests, 20 membranes,* all reagents)
LIS-340-000 Lift kit food (20 tests, 20 membranes, all reagents)
LIS-600-100 Lift basic-100 / 100 (100 tests, 100 membranes)
LIS-600-120 Lift E-basic- 100 / 20 (100 tests, 20 membranes)
LIS-600-500 Lift basic-500 / 500 (500 tests, 500 membranes)
LIS-600-510 Lift E-basic-500 / 100 (500 tests, 100 membranes)
LIS-600-520 Lift E-basic-1000 / 200 ( 1000 tests, 200 membranes)
LIS-600-010 Lift E-basic-M / 100 (100 membranes only)
Kit components
LIS-040-100 Stomacher bags with Mesh insert (20)
LIS-040-300 Stomacher buffer concentrate (20 tests)
LIS-040-400 Filters (20)
LIS-040-500 Filtrate collection tubes (20)
LIS-050-300 Capture buffer concentrate (20 tests)
LIS-060-100 Media plates (10)
LIS-060-200 Membrane wash buffer concentrate (20 tests)
Required reusable accessories
LIS-101-000 Glass spreaders (25)
LIS-102-100 Magnetic capture vial rack (20 vial capacity)
LIS-108-000 Reciprocating shaker
LIS-109-000 Capture vial rotator

*Note: membranes are only needed for analysis of positive samples. Since the majority of samples are usually negative, Geneq sells some test kits with fewer membranes than tests, and passes the savings on to the customer.