GENEQ > Biotechnology > Bacteria Detection
| E*Colite |
E*Colite is a carbohydrate
rich, selective medium particularly adept at resuscitation and detection
of chlorine stressed coliform and E.coli encountered in drinking, bottled
and food/beverage waters. |
| FEATURES |
|
| SIMPLE |
The bag design houses all test components.
Simply add water sample no preparation, no test bottles. The bag also
acts as a sample container. There are no collection bottles or loose sodium
thiosulfate tablets. Reagents are stable up to 12 months at room temperature. |
| PROCEDURE |
Add water sample and seal the bag.
Push the water into the medium compartment. Incubate at 35°C for 28
hours. |
| INTERPRETATION |
If the sample turns blue, coliforms
are present. If a blue coliform positive sample fluoresces, E.coli are
present. If a blue sample does not fluoresce, continue incubating an additional
20 hours (total 48 hours). If blue growth fluoresces after the 48 hours,
the sample is confirmed E.coli. If the blue bag does not fluoresce after
48 hours, the sample is coliform positive and E.coli negative. |
| EASY |
E*Colite uses X-gal indicator to
produce an indigo blue positive reaction. This blue color, as opposed
to traditional yellow color, vastly improves interpretation of turbid
or rust and heterotropic bacteria contaminated waters. All reagents and
containers are self enclosed, reducing space for supplies and eliminating
accessory inventory, tracking and storage. |
| SENSITIVE |
E*Colite detects a single microorganism
in 100ml water and has been demonstrated to have 98% correlation with
the LTB/BGLB method in detecting 4 log chlorine stressed coliform. It
demonstrates a 93% correlation with LTB/ECMUG in detecting 4 log chlorine
stressed E.coli. |
| SPECIFIC |
E*Colite uses selective detergents
to minimize non-specific bacteria interferences. It recovers stressed
organisms more effectively than LTB/BGLB or ECMUG (reference method).
In a 500 sample comparability study with 4 log chlorine stressed coliform,
E*Colite recovered 53% positive coliform while reference recovered 48%.
Stressed E.coli were recovered 57% in E*Colite versus 48% by reference. |
| BIO-SAFE |
| A self enclosed bactericide is provided to introduce a 7-8 log reduction of any grown organisms. The bactericide provides critical bio-safety for operations without access to an autoclave. |
| CAT. NO. | DESCRIPTION |
| ECOLT-25K | Ecolite 25 tests |
| ECOLT-100K | Ecolite 100 tests |
| ECOLT-500K | Ecolite 500 tests |
| ECOLT-5000K | Ecolite 5000 tests |