GENEQ > Biotechnology > HPLC

Fluorescence Detector

Fluorescence techniques are particularly useful in the analysis, by liquid chromatography, of amino acids; DNA, including fragments; enzymes (NAD/NADH); environmental testing (water analysis); pesticides and herbicides; proteins; fermentation processes (sugars); vitamins, steroids, various
drug metabolites; and many more compounds particularly in the biotechnology area. For all these applications the sensitivity and specificity of the DFL-10 makes it an ideal detector for the analysis.

Sensitivity
D-Star’s new flowcell arrangement for the DFL-10 uses a unique excitation and emission geometry. It also minimizes stray light entering into the analysis. Photomultiplier detection provides highly sensitive detection at very low light levels. A completely enclosed optical compartment and a thermally stabilized system enable the instrument to provide detection levels required in fluorescence assays.

Quantitative analytical data can be obtained for many varied fluorescence applications with the DFL-10. The sensitivity of the DFL-10 is enhanced by the selection of precision excitation and emission filters. The correct filter selection offers greater specificity and adds to the analytical accuracy of the assay. The excitation filter is usually a narrow band (N) filter that should be selected as close as possible to the maximum excitation wavelength of the compound under analysis.

The emission filter is typically a “Cut-On” (C) filter with a wavelength value that is approximately 10nm less than the maximum emission wavelength
for the molecule. “Cut-On” filters pass all wavelengths of light greater than the “Cut-On” wavelength value.

Once you have selected the base configuration, select the appropriate lamp, excitation, and emission filters. The lamp, excitation and emission filters are ordered separately and not included in the base price.