GENEQ > Biotechnology > Fluorimeters

6280 fluorimeter 190-650nm
High sensitivity photomultiplier detector Wavelength range 190 - 650nm Raw fluorescence and concentration display Kinetic (timed readings) function

Vitamin A deficiency can affect eyesight The high sensitivity of fluorimetric measurement techniques enables trace levels to be determined in a broad range of samples. Selectivity is also enhanced enabling interference free analysis of even the most complex sample matrix. All models in the 62 Series enable simple raw fluorescence measurements to be made without the need to calibrate. These comparative fluorescence levels can be used as a basic indicator or for method evaluation procedures. Precise concentration measurements can be made by calibration with a blank and standard solution. Where expected sample concentrations may cover a wide range a calibration curve can be created, using up to six standards. Twenty methods can be created and saved with both a numeric ID as well as an alphanumeric name. By entering the excitation and emission wavelengths in the method the filter characteristics and positions are automatically verified before measurements are made. The model 6280 is ideal for a broad range of applications in food and beverage analysis, including Histamine, Quinine, Aflatoxins and Vitamins. It also has a broad range of uses in Biochemistry and Life Science analysis with measurement of parameters such as DNA/RNA, Protein, Steroids, Enzymes and Drugs. For water and environmental analysis it has applications for Dye Tracing (Flow Measurement), Cyanobacteria and Ammonium measurement amongst many others.

6285 fluorimeter 190-850nm
Red-enhanced photomultiplier detector Wavelength range 190 – 850nm Calibration with up to 6 standards Method lock with password access
Increasing levels of algae Where applications generate an Emission wavelength between 650 and 850nm, and the high sensitivity of a photomultiplier is still required, the model 6285 will fit the bill. The measurement of Chlorophyll a, Algal Pigments and Rhodamine B are just three examples of where this enhanced wavelength range will be required, while all the general applications can still be carried out. Like all models in the 62 Series the 6285 can display concentration values based on a calibration with one to six standards. A simple step-by-step procedure enables a calibration curve to be generated hence the accurate determination of sample concentrations over a wide range. When working in the simple Raw Fluoresence mode the Auto-Set Gain function ensures the optimum reading resolution is achieved by measuring the fluorescence produced by the highest standard/ sample and automatically setting this to 95% of the full range. On all of the models up to 20 methods can be created, then stored for future use. These methods can be locked so change or modification cannot be made without the entry of a security PIN code. To further conform to GLP an operator ID and calibration reminder can also be set. For temperature sensitive assays the optional electrically heated cuvette holder can be used.

6270 fluorimeter 190-1100nm
Rugged photodiode detector Wavelength range 190 – 1100nm Time and date stamped results High quality, low cost measurement system

DataWay PC software & accessories

Software DataWay gives you unlimited storage of results and graphs in files on your PC – by far the best and most secure way to store your personal data when working on a multi-user instrument. Because DataWay is so easy to use you won’t hesitate to choose it for even the simplest tasks. Just press the Print key on the instrument or click the ‘Take reading’ button to transfer your data to the PC. Work with your data off-line with instant access to your nominated DataWay files through a single click on the Load icon. If you want your data in an ExcelTM spreadsheet click the copy option and select ExcelTM, to automatically open it with your data pasted in the cells; if you are working with another programme you can still copy to the clipboard and then paste the data where you want it. DataWay has its own powerful graphing function that enables multiple overlays of comparative data as well as the live up-date of data when continuous sampling is enabled (from the PC or instrument). High and low alarm levels can also be displayed with incident reports when the graph crosses these thresholds. With its flexible print-out options DataWay will often be the only tool you need to record and present your results from the 62 Series Fluorimeters, and many other Jenway instruments too.

Accessories

Where portability or field analysis is important all models in the 62 Series can be fitted in a tough carry case, enabling the instrument and a range of accessories to be carried easily and securely with a reduced risk of losing or misplacing items when working in awkward or in-hospitable situations. All models can be powered from any mains supply (100 to 230Vac) as well as a 12 Vdc supply. When being used in a vehicle an optional supply cable with a vehicle auxiliary socket (cigarette lighter type) can be used, a number of independent battery packs are also available.

Sampling options & accessories
The programmable peristaltic sipper pump can be adjusted by the operator to inject a repeatable volume of sample/standard into the chamber of a flow-through cuvette. An air gap to separate samples may be selected as well as a wash cycle and the option to pump through the cuvette to waste or return the sample to its original container after measurement. The programme can also be overridden to pump sample continuously through the cuvette for monitoring a bulk or flowing sample over a period of time. Fluorescence intensity is inversely proportional to temperature so care must be taken when comparing samples measured at different ambient temperatures. Temperature can also affect other properties of the samples to be measured; this may further influence the accuracy or reproducibility of results, just one example being the rate of enzyme kinetic reactions. The electrically heated cell holder with its digital control unit ensures samples are always at the same temperature when measured, removing this potential variability.
	Sipper Pump
	Heated Cell System (heated cell holder not shown)

Bandpass Filters
627 126	UG1	320-380nm
627 124	BG28	80-500nm
627 125	VG9	480-580nm
Cut-off Filters (low cut, transmission blocked below stated wavelength)
627 127	Kodak 29	610nm
627 128	Ilford 201	545nm
627 129	Kodak 8	475nm
627 130	Kodak 2B	395nm
627 131	Glass	305nm
Interference Filters
627 132	Wavelength 254nm
627 133	Wavelength 340nm
627 134	Wavelength 380nm
627 135	Wavelength 400nm
627 136	Wavelength 470nm
627 137	Wavelength 514nm
627 138	Wavelength 365nm
627 139	Wavelength 450nm
Serial Printer
A 40 column serial printer is available for use with the 62 Series Fluorimeters
037 701	Serial printer supplied with roll of thermal paper, protective pouch, serial connection lead, power supply and three power connection leads.
037 702	Paper roll, thermal	Serial Printer
037 801	Interface cable kit

Technical Specifications
Model	6270	6280	6285
Light Source	Pulsed xenon lamp with press-to-read operation	Pulsed xenon lamp with press-to-read operation	Pulsed xenon lamp with press-to-read operation
Expected Lifetime	No replacement required in normal operation	No replacement required in normal operation	No replacement required in normal operation
Sensitivity (Quinine Sulphate)	1µg/ml	<1ng/ml	<1ng/ml
Dynamic Range	5.2 x 10(4)	5.2 x 10(6)	5.2 x 10(6)
Detector	Photodiode	Photomultiplier	Photomultiplier
Wavelength Range	190 to 1100nm	190 to 650nm	190 to 850nm
Wavelength Selection	Intelligent Filter Modules (IFM)	Intelligent Filter Modules (IFM)	Intelligent Filter Modules (IFM)
Raw Fluorescence	Yes	Yes	Yes
Concentration	Yes	Yes	Yes
Calibration	Blank + up to 6 standards	Blank + up to 6 standards	Blank + up to 6 standards
Kinetics (Timed Readings)	In both above modes	In both above modes	In both above modes
Time Limits	1 reading every 3 to 999 seconds	1 reading every 3 to 999 seconds	1 reading every 3 to 999 seconds
Methods Stored	20	20	20
Results Stored	100/method	100/method	100/method
GLP	Method lock with password access Time and date stamped results Last calibration date and time stored Calibration interval set Calibration due reminder User ID	Method lock with password access Time and date stamped results Last calibration date and time stored Calibration interval set Calibration due reminder User ID	Method lock with password access Time and date stamped results Last calibration date and time stored Calibration interval set Calibration due reminder User ID
Outputs	RS232 Serial Port	RS232 Serial Port	RS232 Serial Port
PC Software	Compatible with DataWay (option)	Compatible with DataWay (option)	Compatible with DataWay (option)
Sample Handling	10mm square cuvette holder (standard)	10mm square cuvette holder (standard)	10mm square cuvette holder (standard)
Heated Sample Holder (Option)	Yes	Yes	Yes
Sipper Pump (Option)	Yes	Yes	Yes
Power Supply	100 to 240Vac universal mains adapter 12Vdc input (cigarette lighter cable and battery pack options)	100 to 240Vac universal mains adapter 12Vdc input (cigarette lighter cable and battery pack options)	100 to 240Vac universal mains adapter 12Vdc input (cigarette lighter cable and battery pack options)
Size	365 (w) x 272 (d) x 160 (h) mm	365 (w) x 272 (d) x 160 (h) mm	365 (w) x 272 (d) x 160 (h) mm
Weight	6Kgs	6Kgs	6Kgs