Temperature Controlled Tissue Baths

for the Vibroslice

The 765LP and 765HP Temperature Controlled Tissue Baths are designed for use with the tissue cutters models HA752 and MA752. Research has shown that unfixed brain slices sectioned at 4°C give better tissue preservation and are viable for longer in-vitro recordings. Additionally, some enzyme histochemical techniques give better staining results when sectioned at low temperatures.

The bath assembly will fit onto a Vibroslice without modification and the bath chamber has the same volume as the standard tissue bath (752/2B). The tissue holder is the standard tissue holder (752/2A).

Current from the power supply flows through one side of the thermoelectric elements, which act as heat transfer units. Heat is drawn off, cooling the solution in the tissue bath. The heat generated by this process is removed by the water supply fed through the heat exchanger. The unit uses proportional temperature control.

This will take the bath temperature to within 0.5°C at the point of measurement. The temperature feedback sensor is located in the stainless steel temperature feedback block (the high power 765HP has an additional heatsink block) and will indicate the temperature at the point of measurement. There will, however, be a small variation in temperature vertically through the bath. Experience will show the best temperature to be set for any given requirement and ambient temperature.

SPECIFICATIONS
Display Resolution	0.1°C
Temp. Accuracy	±0.1°C
Temp. Range 765LP	+40 to -1°C
Temp. Range 765HP	+40 to -10°C
Note that the actual temperatures achievable will be dependent upon the solutions used and local temperature conditions
Voltage Requirements LP (HP)	230V, 50 Hz fused at 1A, (2A) 110V, 60 Hz fused at 2A, (4A) Set via swich on rear of unit
Power Requirement LP (HP)	250W (350W)
Observation Accessories
752LS Cold Light Source	20W/12V halogen lamp, 2000 hour life approx. Fan and electronic power supply Adjustable with potentiometer Twin 500 mm fibre optic light guides
752IM Inspection Microscope	Binocular, 45° inclined x1 to x4 zoom x10 to x40 magnification WFx10/20 mm eyepiece 80 mm working distance

CAT. NO.	DESCRIPTION
CA765LP-00	Model 765LP, Low power temperature controller
CA765HP-00	Model 765HP, High power temperature controller
CA752LS-00	Model 752LS, Free standing cold light source
CA752IM-00	Model 752IM, Free standing inspection microscope
Note: A range of acessories and consumables including autoclavable cutting heads and tissue baths are also available.