GENEQ > Biotechnologie > Coupe de Tissu
| Microtomes oscillants |
| modèle Vibroslice™ |
| The manual tissue transport, model HA752 | |
|
The manual advance of the section gives complete control
during sectioning. The HA752 is recommended for use in producing a small
number of sections or for use on tissues with differing mechanical properties.
Temperature controlled tissue baths for this model is sold separately CLICK HERE for specifications |
| The motorised tissue transport, model MA752 | |
|
Control of the speed of advance of the tissue to be sectioned
is set on a rotary dial and the user then presses a footswitch leaving
both hands free during operation. This model is recommended for use in
cutting serial sections for reconstruction or for producing multiple sections.
Both the HA752 and the MA can be fitted with the tissue both and holder and a cutting head that can be removed for autoclaving in applications where sterility is important. One of the new features on the latest Vibroslice is the variable blade angle. A protractor is provided and the angle is set by simply slackening the clamping screw positioning to the required angle and retightening. As a general rule the softer the tissue the steeper the angle is required for optimum slice integrity. Temperature controlled tissue baths for this model is sold separately CLICK HERE for specifications |
| The remote motorized, model ROMA752 | |
|
This machine was design especially for work with hazardous tissues such as those containing Prions or rabies infection. The cutting head, tissue bath and associated drive mechanisms are operated on an umbilical from the control box and thus can be placed inside a controlled environment such as a thin film isolator. A tissue bath and cutting head capable of withstanding autoclaving at 140°C are included with this machine. |
| DESCRIPTION |
|
The instrument has been designed to cut 50 to 700 micron sections of fresh, unfixed tissue for in vitro recordings, tissue culture and microassay work. It can also be used to section fixed tissue down to 20 microns without previous embeddind or freezing. The Vibroslice has also been redesigned and considerably upgraded in its performance form the original specification and will section tissue such as brain stem, and liver maintaining the integrity of surface cells for recording. A vibrating blade slices tissues without the traumatic damage of other methods. The blade oscillates at a fixed lateral displacement of 1,0mm at a frequency set by the user and then actuated by a footswitch. If damage should occur to the blade the lateral displacement minimizes scoring of the tissue. Both frequency of the blade oscillation and the rate of advance of the section are important in producing good quality sections. The Vibroslice is designed to be flexible in its operation and has a removable tissue holder and bath, which allows tissue to be prepared and stored ready for later use, provides easy cleaning and prevents carryover of fixative between fixed and unfixed tissue. Recent product design has incorporated a variable angle blade holder for the first time. The advance speed on the motorized models is now ultra-low being half of the previous model and the speed of oscillation has been raised again. Changing of drive belts is now much easier with the readily removble molded cover. All models are supplied with a standard tissue bath and holder and 10 stainless steel blades. The Vibroslice has been designed for many years of trouble free operation, particularly with sealing against the ingress of physiological fluids. Nevertheless regular checking of the moving parts and servicing by a skilled engineer are recommended to maintain the very optimal performance. |
| SPECIFICATIONS | |
| Section Thickness | |
| Minimum | Fixed tissue 20 microns Fresh tissue 50 microns |
| Maximum | 700 microns |
| Step Size | 5 microns |
| Tissue Bath | 55 x 82 x 31 mm in dimensions and 140 ml volume |
| Bath Advance | |
| Model HA752 | Manual |
| Model MA752 | Motorized with variable speed from 0.07mm/second |
| Model ROMA752 | Motorized with variable speed from 0.07 mm/second |
| Bath Return | |
| Model HA752 | Manual |
| Model MA752 | Fixed speed motorized |
| Model ROMA752 | Fixed speed motorized |
| Section Thickness | |
| Model HA752 | Manual |
| Model MA752 | Manual |
| Model ROMA752 | Dual speed motorized |
| Vibration Speed | User adjustable with handsfree operation in all models |
| Blade Displacement | 1 mm nominal in all models |
| Power supply | 230VAC, 50 Hz or 115VAC, 60 Hz |
| Power Rating | HA752, 15W MA752 and ROMA752, 20W |
| CAT. NO. | DESCRIPTION |
| CAHA752-00 | Model HA752 with manual tissue transport |
| CAMA752-00 | Model MA752 with motorized tissue transport |
| CAROMA752 | Model ROMA752 with motorized tissue transport and remote operation |