GENEQ > Biotechnology > Microinjectors
Programmable 4-Channel Pressure Injector
Model PM 2000B


| FEATURES |
|
The PM 2000(B) Programmable 4-Channel Pressure
Injector is designed for intracellular injection and extracellular perfusion.
It can be used for directly injecting different molecular agents, labelled dyes,
genes, RNA, DNA, proteins and cell organelles to a cell, or performing a perfusion
of up to 15 combinations from four different agents and drugs to a cell or a
group of cells. It is also a superior device for intracytoplasmic sperm injection
and cell electrophysiology. By combining an advanced microcontroller and precise
pneumatic components, the PM 2000(B) can simultaneously control four injection
micropipettes, one cell holding pipette and one drain pipette.
The four injection micropipettes can be controlled separately
or combined together to perform actions such as injection, capillary action
balancing, suction or clear up. The cell holding pipette can produce an adjusted
vacuum to hold a cell or eject a pressure to push a cell or just clear the holding
pipette itself. Also, the drain pipette can produce an adjusted vacuum to drain
out liquid precisely and synchronize with every microperfusion. The PM2000(B)
can deliver different agents and drugs from picolitters to continued perfusion.
There are individual regulators for adjustments of suction vacuum,
cell holding vacuum, drain vacuum, inject pressure and balance pressure. A LCD
window displays full information such as pressure, vacuum, time, sequence steps
and actions.
During pressure mode, all pressure readings are simultaneouslyreal-time
displayed on the LCD screen so that the user can conveniently preset all the
pressures without switching the pressure measurement. Meanwhile, previous pressure
reading can be recovered on the LCD for further comparison.
The PM 2000(B) provides programmable timers and counters to
deliver accurate timing and counting for corresponding functions.
The PM 2000(B) allows the user to program custom-designed action
sequences. There are 16 savable sequences with 32 programmable steps in each
sequence. The sequences can be used to perform and repeat different injections
and perfusion cycles precisely. Also, the sequential cycles can be automatically
performed or triggered each step by manual. Even the interval time between steps
is programmable for automatic sequential cycle.
All preset timers, counters and sequential steps are saved even
turn off the power. By pressing a button, even the pressure readings can be
saved too. Therefore, the PM 2000(B) is complete reproducible for an experiment
at anytime.
An attached foot switch can control all preset step or be used
as an injection trigger. An optional remote keyboard gives the user another
convenient tool to control the whole operation. The exceptional versatility
and extremely precise control are the hallmark of the PM 2000(B).
|
SPECIFICATIONS
|
|
|
Output Channels
|
Eight injection / perfusion
outputs, one synchronized drain out and one cell hold channel
|
|
Input Gas Pressure
|
30 - 100 psi
|
|
Clearing Pressure
|
Same as input pressure
|
|
Clear Hold Pressure
|
0-3 psi (regulated)
|
|
Injection Pressure
|
0.05 - 60 psi ( regulated
)
|
|
Balance Pressure
|
0.05 - 10 psi ( regulated
)
|
|
Fill Vacuum
|
0" - 24" Hg (
regulated )
|
|
Hold Vacuum
|
0" - 30" of water
(regulated )
|
|
Drain Vacuum
|
0" - 30" of water
( regulated )
|
|
Repeatability
|
+/- 0.02 psi
|
|
Display Accuracy
|
+/- 0.05 psi for pressure,
+/- 0.2" for vacuum (<2" H2O).
|
|
Timer Setting Range
|
0 msec - 327.67 sec. (10
msec. Resolution for whole range)
|
|
Counter Setting Range
|
1 - 255
|
|
Savable Sequences
|
16 sequences
|
|
Programmable Steps
|
32 steps for each sequence.
|
|
Remote Control Ports
|
DB9 connector for remote
key pad, foot switch jack and BNC for negative TTL trigger
|
|
Signal Output Ports
|
Four BNCs for injection
signal on output port 1-4
|
|
Power Consumption
|
100, 120 or 220 VAC, 35
Watts
|